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The mechanism of action of estrone (E1) on gonadotropin and prolactin (PRL) secretion was examined in pituitary cell cultures. The basal and stimulation by CRF of LH and FSH released into the culture media was inhibited by 0.1 mM E1. The PRL content of the culture media increased in response to CRF. E1 did not affect the PRL release. The E1 inhibition of FSH release was potentiated by the simultaneous treatment with 0.1 mM E1 and with 5-10 microM E2. E1 did not affect the CRF-induced increase in FSH release. E1 did not inhibit the CRF-induced increase in PRL release. In the case of LH secretion, E1 decreased the CRF-induced increase in LH secretion. The inhibition of basal LH secretion and the inhibition of LH response to CRF were increased by 1-20 microM estradiol (E2). The inhibitory effect of E2 on LH secretion was reduced by 5-10 microM E1. The inhibitory effect of E1 on LH release was potentiated by 2-20 microM E2. These results suggest that E1 acts on gonadotropin release via E1 receptors in the anterior pituitary. E1 inhibited the CRF-induced gonadotropin release, and the inhibitory effect of E1 on LH release was potentiated by E2. E1 inhibited the CRF-induced PRL release, and this inhibitory effect of E1 on PRL release was not potentiated by E2.Office of Children's Ombudsman
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